http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107365738-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2517-10 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0604 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-073 |
| filingDate | 2017-08-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-02-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-02-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-107365738-B |
| titleOfInvention | Method for preparing cow and cattle xenogenesis in-vitro fertilization embryo |
| abstract | The invention discloses a method for preparing in vitro fertilization embryos of dairy cows and cattle. In the method, discarded cow ovaries in a slaughterhouse are washed by temperature-increasing physiological saline in a gradient manner, oocytes in the cow ovaries are extracted for in-vitro maturation culture, and high-quality in-vitro maturation culture oocytes are obtained; after the sperms of the high-yield cows are unfrozen, the high-yield cows are placed into the fertilization liquid and lightly blown by a liquid transfer gun, and then the high-yield cows and the fertilization liquid are inverted up and down for several times, and float up in an incubator for in vitro fertilization, so that the high-yield cows and cattle in vitro fertilization embryos are prepared. According to the invention, the ovaries discarded in the slaughterhouse are adopted to obtain the oocytes, so that the utilization rate of cow resources is improved. And better effect is obtained through the improvement of technical details. The utilization of the invention can greatly improve the utilization rate of the male livestock with excellent breeds, and the male livestock with excellent breeds can obtain a large number of offspring, thereby expanding the function of the male livestock in herd genetic improvement. |
| priorityDate | 2017-08-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 79.