| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_cb351a5a4e03ee3773bd0df5dbae9ac3 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12R2001-40 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N13-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-01
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P17-12
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-205 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-40 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-20
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N13-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P17-12
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-01 |
| filingDate |
2017-09-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_80ed89b5e253b2c4d7f7ad380134ffdf
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_83ff2e563253a26dc0a59b4d2587199e
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3a857423d5bbe4246d44c10cf6c3a6a6
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9f6c75b2ee13e7f08aa468e9d3399105
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0e3800ea59bc21a91e6fc180806a3986
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_96518cbef0d89a0bd5d61785f8a88aa9
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0e535e446622ce4c879cdc5456b1be6c |
| publicationDate |
2017-11-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
CN-107365726-A |
| titleOfInvention |
A kind of the nitrilase producing strains mutant and its selection of high nitrile substrate tolerance |
| abstract |
It is an object of the invention to provide a kind of combination ARTP mutagenesis and the method for the production nitrilase pseudomonas putida mutant of the micro enzyme reaction method high flux screening nitrile substrate tolerance liftings of OPC TCA, belong to industrial biotechnology field.Through one plant mutant body mut D3 of the micro enzyme reaction primary dcreening operations of OPC TCA, shaking flask secondary screening and the final acquisition of passage, in the case of higher Final substrate concentrations are 125mM, the substrate tolerance of the bacterial strain significantly improves, the nitrilase enzyme activity of simultaneous mutation body is 1.51 times of wild mushroom, and enzyme activity still keeps stable after 20 passages.The cyanopyridine of mutant strain energy Efficient Conversion 3 synthesizes nicotinic acid, and nicotinic acid cumulative production significantly improves compared to wild mushroom.The present invention complies fully with mutagenesis and the screening needs of industrialization nitrilase producing bacterial strain, and can be applied to the biosynthesis of nicotinic acid, has important application value. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111733192-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108486209-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108486209-B
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108823098-A |
| priorityDate |
2017-09-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |