| abstract |
The invention discloses a kind of preparation method of autologous three skin fibroblasts preparation, the autologous three skin fibroblasts preparation is made up of platelet rich plasma, autologous collagen concentrate and fibroblast；Platelet rich plasma：Autologous collagen concentrate=1:4, autologous fibroblasts concentration is (1.0~2.0) × 10 7 /ml.The preparation method of the autologous PRP skin fibroblasts preparation comprises the following steps：Human skin fibroblasts separation, culture；The preparation of autologous high concentration collagen liquid；The preparation of autologous platelet rich plasma；The preparation of autologous three skin fibroblasts parenteral solution.It is autologous that invention formulation active ingredient all belongs to client, and during Fibroblast cell-culture, with autoserum medium culture, be truly realized safely, effectively, individuation the characteristics of.Acted on available for skin anti-wrinkle, scar reparation, regeneration etc., solve the problems, such as skin ageing. |