abstract |
The present application relates to cysteine engineered antibodies and conjugates. Preparing a cysteine engineered antibody comprising free cysteine amino acids in the heavy or light chain, i.e. mutagenizing the nucleic acid sequence of a parent antibody and replacing one or more amino acid residues with cysteine, so as to encode the cysteine engineered antibody; expressing the cysteine engineered antibody; and isolating the cysteine engineered antibody. Certain highly reactive cysteine engineered antibodies were identified by the PHESELECTOR assay. The isolated cysteine engineered antibody may be covalently attached to a capture label, a detection label, a drug moiety, or a solid support. |