http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107326093-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-158 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6883 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6851 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6883 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6851 |
| filingDate | 2017-09-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-01-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-01-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-107326093-B |
| titleOfInvention | Taqman probe-based fluorescent quantitative PCR (polymerase chain reaction) one-step kit for detecting genes related to chronic kidney diseases and application thereof |
| abstract | The invention discloses a Taqman probe-based fluorescent quantitative PCR (polymerase chain reaction) one-step kit for detecting genes related to chronic kidney diseases and application thereof, wherein the kit comprises upstream and downstream primers and probes for detecting VIM, BCL2, PODX and TGFB1 genes, and further comprises upstream and downstream primers and probes for detecting B2M reference genes. The fluorescent quantitative PCR kit has the advantages of small material taking amount, simple and convenient operation and short detection period, and can be used for quickly detecting various related disease genes of chronic kidney diseases. Meanwhile, mRNA reverse transcription and fluorescence quantification are completed in one step in the detection process and are performed in a single tube without opening a tube cover, and the generation of a fluorescence signal is not only strongly dependent on the hybridization of a target template and a probe, but also strongly dependent on the amplification of the target template, so that the phenomenon of non-specific amplification does not exist. Thereby being capable of rapidly, specifically and sensitively detecting the mRNA expression level of the chronic kidney disease related gene. |
| priorityDate | 2017-09-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 62.