http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107312107-B
Outgoing Links
Predicate | Object |
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classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C08B37-0003 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C08B37-006 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C08B37-00 |
filingDate | 2017-08-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2019-03-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2019-03-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-107312107-B |
titleOfInvention | A kind of technique that enzymatic isolation method extracts Dendrobium officinale polysaccharide |
abstract | The invention discloses the techniques that the enzymatic isolation method of a kind of efficient, safety, suitable industrialized production extracts Dendrobium officinale polysaccharide.It mainly comprises the steps that and takes dendrobium candidum, it crushed 20-80 mesh, 4-6 times of amount is added and contains pectase: cellulase: the weight ratio of laccase (1-2:1-3:0.5-2.5) is mixed and made into the glacial acetic acid buffer solution of compound enzyme system, and (ice acetic acid solution adjusts pH value to 4.0-6.0), 25-50 DEG C of controlled enzymatic hydrolysis temperature digests 1-5h.It is transferred in appropriate vessel, adds 4-8 times of drinking water, refluxing extraction 2h, filter (120 mesh), filtrate is concentrated into relative density 1.25-1.40 (60 DEG C);Ethyl alcohol, which is added, makes medical fluid alcohol content up to 70-80%, stirs evenly, and after standing 12h, centrifugation collects sediment, flings to ethyl alcohol;60-80 DEG C vacuum drying to get.Polyoses content and mannose content are measured respectively and calculates recovery rate, and as a result Dendrobium officinale polysaccharide recovery rate is 39.88%-46.16%, and mannose recovery rate is 48.26%-55.63%. |
priorityDate | 2017-08-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 98.