http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107312088-B

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classificationCPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-10
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http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-577
classificationIPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-10
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http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-577
filingDate 2017-05-24-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2020-09-01-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2020-09-01-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-107312088-B
titleOfInvention Porcine epidemic diarrhea virus specificity SIgA ELISA detection kit and application thereof
abstract The invention discloses a Porcine Epidemic Diarrhea Virus (PEDV) specific SIgA ELISA detection kit and application thereof. The kit comprises a monoclonal antibody of an anti-SIgA SC fragment, wherein the monoclonal antibody is secreted and generated by a microbial hybridoma cell strain 2F9, the hybridoma cell strain 2F9 is preserved in the China general microbiological culture Collection center, and the microbial preservation number is as follows: CGMCC No. 13813. According to the invention, SIgA purified from colostrum is used as an antigen, a BalB/C mouse is immunized, a hybridoma cell strain which stably secretes SIgA molecular secretion tablet (SC) is obtained, and an ELISA method and a kit for detecting PEDV specific SIgA antibody are established. The invention provides an effective technical means for further researching the specific SIgA level and mucosal immunity evaluation of pigs infected with PEDV and immunized with PEDV vaccines.
priorityDate 2017-05-24-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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