http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107300594-B
Outgoing Links
Predicate | Object |
---|---|
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-06 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-02 |
filingDate | 2017-07-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2019-10-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2019-10-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-107300594-B |
titleOfInvention | The method that liquid chromatography mass combination directly detects amino acid in biological tissue's Uniform Sample |
abstract | The invention discloses the methods that a kind of combination of liquid chromatography mass directly detects amino acid in biological tissue's Uniform Sample, including handling sample with extracting solution, the extracting solution by liquid chromatogram flowing phase composition, including acetonitrile and buffer, the concentration of acetonitrile is 50%-95%, the ammonium formate that the formic acid and concentration that buffer is 0.1%-0.3% by concentration are 1-10mM forms, or the ammonium acetate that the acetic acid and concentration for being 0.1%-0.3% by concentration are 1-10mM forms, and the pH of the extracting solution is between 3-5.5.Method disclosed by the invention can directly detect the biological tissues such as blood, urine and cerebrospinal fluid Uniform Sample, sample processing method is simple, sample dosage is few, can be down to 4 μ l, and detection sensitivity is high, up to pg/ml grades, detectable amino acid concentration range is wide, and matrix interference is small, and accuracy in detection is high, up to 29 kinds of the amino acid classes that can be detected simultaneously, and detection time is short. |
priorityDate | 2017-07-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 56.