| abstract |
The present invention relates to a kind of preparation of the cell climbing sheet component of detection MOG IgG CBA kits, CBA detection kits and its application, the preparation method of cell climbing sheet component is：S1：MOG plasmid constructions；S2：It is overexpressed the slow virus packaging of MOG genes；S3：The cell line (MOG 293T cell lines) for being overexpressed MOG genes is built；S4：The preparation of cell climbing sheet component.The kit includes cell climbing sheet component, anti-human igg FITC secondary antibodies, confining liquid, PBS and mountant；The detection of clinical sample MOG autoimmunities IgG antibody can be carried out using the kit.The kit of the present invention is simple to operate, the high specificity of detection, and sensitivity is high, and experimental result is reproducible, can carry out popularization and application, and available for the detection of clinical sample MOG autoimmunity IgG antibodies. |