| Predicate |
Object |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2267-03
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2310-20
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2227-105 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-22
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K67-0275
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K67-0276
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y108-01008
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-902
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-907
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1137
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0051 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01K67-027
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-90
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-22 |
| filingDate |
2017-05-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2020-03-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate |
2020-03-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
CN-107287245-B |
| titleOfInvention |
A Construction Method of Glrx1 Gene Knockout Animal Model Based on CRISPR/Cas9 Technology |
| abstract |
The invention discloses a method for constructing a Glrx1 gene knockout animal model based on CRISPR/Cas9 technology. It includes the following steps: 1. Selection and design of gRNA targeting mouse Glrx1 gene. 2. Construction of sgRNA vector. 3. In vitro transcription of sgRNA. 4. One-cell injection of fertilized eggs in mice. 5. Birth and identification of F0 generation mice. 6. The positive F0 generation mice were bred, and the F1 generation mice were born and identified. By the method of the present invention, the Glrx1 gene knockout animal model can be successfully obtained. |
| priorityDate |
2017-05-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |