http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107287185-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01H4-001 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01H4-008 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01H4-005 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H4-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-05 |
| filingDate | 2017-07-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-05-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-05-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-107287185-B |
| titleOfInvention | Protoplast fusion method of potato tetraploid cultivar and diploid wild species |
| abstract | The invention discloses a method for fusing potato tetraploid cultivars and diploid wild species protoplasts, which is characterized in that a set of optimal conditions for separating and purifying test-involved potato protoplasm, a protoplast culture medium, a callus regeneration culture medium and a tetraploid and diploid protoplast fusion method are obtained by searching and optimizing a set of protoplasts obtained by separating and purifying potato protoplasm by adopting an enzymolysis method and an interface method and fusing the tetraploid and diploid protoplasts by adopting a polyethylene glycol (PEG) induction fusion method. In a fusion experiment of Fiurodebuit and wild diploid materials (E12 and E25) carried out by using the method, 2 fusion strains and 4 fusion calluses which are detected by SRAP molecular markers through DNA are obtained; these materials can be used as subsequent experimental materials to determine the variation of the agronomic traits and the possible applications in production of the obtained fusion lines. |
| priorityDate | 2017-07-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 74.