http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107271422-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6402 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 |
| filingDate | 2017-08-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-08-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2019-08-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-107271422-B |
| titleOfInvention | A method of it is separated based on excitation-emission spectrum while measuring the ratio between the ratio between Acceptor-Donor quantum yield and extinction coefficient |
| abstract | The present invention discloses a kind of method for separating based on excitation-emission spectrum while measuring the ratio between Acceptor-Donor quantum yield with the ratio between extinction coefficient, belongs to FRET detection technique field.This method comprises the following steps: the excitation-emission spectrum S of 2 kinds of measurement or two or more receptor number is identical as donor number ratio and FRET efficiency is different donor-acceptor concatenation sample DA[i] , and by S DA[i] Linear separation, which is carried out, according to three excitation-emission spectrum basic vectors obtains three weight factor W D[i] , W A[i] And W S[i] ；With W A /W D For independent variable, W S /W D For dependent variable, linear fit W A[i] /W D[i] 、W S[i] /W D[i] , the slope and n of linear equation A /n D Product inverse be K A /K D , the absolute value of intercept is Q A /Q D .Measurement process of the present invention is simple, time of measuring is short, measurement result is stable and is suitable for different detection systems. |
| priorityDate | 2017-08-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 14.