http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107267549-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2509-00 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8206 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-82 |
| filingDate | 2017-07-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-01-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-01-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-107267549-B |
| titleOfInvention | Method for separating, purifying and efficiently converting mesophyll protoplast of taxus chinensis variety 406 |
| abstract | The invention discloses a method for separating, purifying and efficiently converting mesophyll protoplasts of a taxus chinensis variety 406, which comprises the following steps: 1) taking the young leaves of the tissue culture seedlings which grow well for 30 days of the Chinese fir as materials, and carrying out dark pretreatment on the materials for 30 hours; 2) separating and purifying mesophyll protoplasts; 3) extracting 50-80 mu g of plasmid from 15-20 mL of overnight culture bacterial liquid, wherein the plasmid is a vector p2FGW7.0; 4) PEG-mediated protoplast transformation. The method for separating, purifying and efficiently converting the mesophyll protoplast of the taxus cuspidata variety 406 is simple and easy to operate, and can achieve the plasmid conversion rate of up to 80 percent in addition to obtaining a large amount of complete and pure mesophyll protoplasts of the taxus cuspidata. Therefore, the problem that the gene function of the sequoia intermedia cannot be deeply researched is solved, certain reference and reference are provided for establishing a transient expression system of other conifer protoplasts, and the method has important application value in the field of forest genetic engineering. |
| priorityDate | 2017-07-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 97.