http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107266583-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2310-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-035 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-215 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-113 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-63 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K19-00 |
| filingDate | 2017-06-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-02-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-02-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-107266583-B |
| titleOfInvention | Targeted activation gene transcription method based on halophilic and alkalophilic bacillus grignard |
| abstract | The invention discloses an NgAGO-VP64 fusion protein, a guide DNA (gDNA) and a method for targeted activation of transcription of an endogenous gene based on Bacillus halophilus Geiger Argonaute (NgAGO). The sequence of the NgAGO-VP64 fusion protein is shown in SEQ ID NO.1 or SEQ ID NO. 2. The guide DNA is any one or a mixture of several of sequence fragments shown in SEQ ID NO. 3-12. The NgAGO-VP64 fusion protein and the targeting DNA are used for co-transfecting cells with transfection reagents, so that the transcription of endogenous genes can be targeted and activated. The invention proves that NgAGO has the capability of combining with guide DNA and recognizing target genomic DNA. The fusion protein constructed by NgAGO and VP64 is found to have the ability of activating endogenous genes in cells for the first time. The invention solves the problem of doubtful observation of the application of the NgAGO recently and provides support for the later development of targeted genome editing tools and other tools. |
| priorityDate | 2017-06-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 540.