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publicationDate 2017-10-17-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-107254529-A
titleOfInvention A kit and method for detecting PIK3CA gene mutation in plasma free DNA
abstract The present invention is a test kit and method for detecting PIK3CA gene mutation in plasma free DNA. The kit contains a specific primer pair and a high-temperature-resistant restriction endonuclease; the specific primer includes a sequence such as SEQ ID NO. The sense primer shown in 1 and the antisense primer whose sequence is shown in SEQ ID NO.2; the high temperature resistant restriction endonuclease TspRI endonuclease. The kit and detection method for detecting PIK3CA gene mutation of the present invention can be used to detect PIK3CA gene mutation, which can improve the sensitivity of mutation detection, reduce the amount of samples used, and be useful for clinical use and popularization.
priorityDate 2017-06-27-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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