http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107254460-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-63 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N11-08 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y304-22002 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N11-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N11-10 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N11-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N11-04 |
| filingDate | 2017-06-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-01-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-01-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-107254460-B |
| titleOfInvention | Carboxymethyl chitosan/sodium alginate composite sponge immobilized papain and application thereof |
| abstract | The invention relates to a carboxymethyl chitosan/sodium alginate composite sponge immobilized pawpaw eggThe preparation method of the immobilized papain comprises the following steps: s1: mixing a carboxymethyl chitosan solution and a sodium alginate solution to obtain a mixed solution for later use; dissolving papain with phosphoric acid buffer solution to obtain papain solution for use; s2: adding a papain solution into the mixed solution under a stirring state, adding a glutaraldehyde solution, stirring for 10-30 min, and then adding CaCl 2 Stirring the solution for 10-30 min to obtain a solidified mixed solution for later use; s3: vacuum freeze drying the solidified mixed solution obtained in the step S2 to obtain the immobilized papain of the carboxymethyl chitosan/sodium alginate composite sponge; in S1, the enzyme activity of the papain solution is 500-700U/mL; in S2, the concentration of the glutaraldehyde solution is 1.0-1.6%. The carboxymethyl chitosan/sodium alginate composite sponge immobilized papain provided by the invention has better enzyme activity recovery rate and stability. |
| priorityDate | 2017-06-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 41.