http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107254458-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-22 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y302-01014 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-26 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-2442 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-815 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-84 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-42 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-81 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-56 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-26 |
| filingDate | 2017-07-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-07-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2019-07-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-107254458-B |
| titleOfInvention | A kind of trichoderma reesei chitinase and its preparation method and application |
| abstract | The invention discloses a kind of trichoderma reesei chitinases and its preparation method and application.The present invention is optimized the chitinase coding gene in trichoderma reesei (Trichoderma reesei), the nucleic acid sequence after optimization is as shown in SEQ ID NO.2 according to the Preference of Pichia pastoris codon.Efficient secretory expression further is carried out using chitinase coding gene of the pichia yeast expression system the optimization, obtains trichoderma reesei chitinase, amino acid sequence is as shown in SEQ ID NO.1.The trichoderma reesei chitinase that the present invention obtains has higher hydrolysing activity to the chitosan substrate of low deacetylation, the crude enzyme liquid that shake flask fermentation generates is the hydrolysis ability with 1mL (protein content about 0.17mg) degradation 0.5g chitosan, and same amount of chitosan of degrading about needs non-specific commercial enzyme 50mg, theoretically efficiency improves 300 times；With good prospects for commercial application. |
| priorityDate | 2017-07-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 433.