http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107245470-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y301-01003 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C11B3-003 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-20 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C11B3-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-20 |
| filingDate | 2017-05-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-12-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-12-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-107245470-B |
| titleOfInvention | Lipase recombinant escherichia coli expression strain, recombinant lipase and application |
| abstract | The invention discloses a lipase recombinant escherichia coli expression strain, recombinant lipase and application, wherein the method comprises the following steps: (1) cloning a lipase GZEL gene onto a pFL-B62cl expression vector to construct a recombinant plasmid; the base sequence of the lipase GZEL gene is shown as SEQ ID NO. 2; (2) and transforming the obtained recombinant plasmid into an escherichia coli Shuffle T7 competent cell, and selecting a positive clone to obtain a recombinant escherichia coli expression strain. And carrying out liquid fermentation by taking the obtained recombinant escherichia coli expression strain as a fermentation strain to prepare the recombinant lipase. The invention has convenient enzyme source, simple fermentation process and low cost; the invention also discloses the application of the enzyme in vegetable oil enzymatic degumming. The phosphorus content in the crude oil can be reduced to below 10mg/kg by degumming the prepared recombinant lipase at 30 ℃ for 3 hours, and compared with the traditional enzyme degumming process, the method can reduce the reaction energy consumption. |
| priorityDate | 2017-05-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 325.