http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107236721-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-22 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-26 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-2434 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y302-01132 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-815 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-14 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-84 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-26 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-42 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-56 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-81 |
| filingDate | 2017-07-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-10-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2019-10-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-107236721-B |
| titleOfInvention | A kind of bacillus subtilis chitosan enzyme and its preparation method and application |
| abstract | The invention discloses a kind of bacillus subtilis chitosan enzymes and its preparation method and application.The present invention is optimized the chitosan enzyme encoding gene of bacillus subtilis according to the Preference of Pichia pastoris codon, and the nucleotide sequence after optimization is as shown in SEQ ID NO.2.Efficient secretory expression further is carried out using chitosan enzyme encoding gene of the pichia yeast expression system the optimization, obtains bacillus subtilis chitosan enzyme, amino acid sequence is as shown in SEQ ID NO.1.The bacillus subtilis chitosan enzyme that the present invention obtains has higher hydrolysing activity to the chitosan substrate of different deacetylations, the crude enzyme liquid that shake flask fermentation generates is the hydrolysis ability with 1mL (protein content 0.3mg) degradation 5g chitosan, and same amount of chitosan of degrading about needs non-specific commercial enzyme 150mg, theoretically efficiency improves 500 times；With good prospects for commercial application. |
| priorityDate | 2017-07-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 367.