http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107227351-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-166 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-156 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6858 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6883 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6883 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6858 |
| filingDate | 2017-06-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-02-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-02-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-107227351-B |
| titleOfInvention | Molecular beacon probe, primer pair and detection method for SNPs sites of GC genes |
| abstract | The invention belongs to the technical field of gene detection, and particularly relates to a molecular beacon probe, a primer pair and a GC gene SNPs site detection method. The molecular beacon probe comprises an upstream sequence, a core sequence and a downstream sequence which are connected in sequence, wherein the core sequence is as follows: 5 '- [ G/T ] GCCACACCCA [ A/C ] -3' or 5 '- [ G/T ] TGGGTGTGGC [ A/C ] -3', the upstream and downstream sequences each comprising: at least three bases that complementarily pair with the GC gene or at least two C or G bases that complementarily pair with the GC gene; the length of the molecular beacon probe is 25-35bp, the Tm value is 62-65 ℃, and the 5 'end and the 3' end are respectively marked by a fluorescence reporter group and a fluorescence quencher group. The molecular beacon probe can cover two SNPs sites of a GC gene, can realize the analysis of GC gene polymorphic sites of a large number of samples, and greatly reduces the detection cost and the analysis difficulty. |
| priorityDate | 2017-06-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 47.