http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107227323-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1007 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P13-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y201-01013 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-865 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-19 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-81 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P13-12 |
| filingDate | 2017-07-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-07-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-07-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-107227323-B |
| titleOfInvention | Construction and application of high-yield selenomethionine saccharomyces cerevisiae |
| abstract | The invention discloses construction and application of high-yield selenomethionine selenium-rich saccharomyces cerevisiae. The target fragment containing the strong promoter is combined with the yeast genome by homologous recombination MET6 Exchanging promoter region of gene to obtain MET6 An overexpression strain. The invention obtains the saccharomyces cerevisiae by screening MET6 The mutant strain SZ1 was overexpressed. Then the mutant strain is used for selenium enrichment operation, the mutant strain is cultured to a stable stage under the induction condition of galactose, and Na with the final concentration of 2.5mM is added 2 SeO 3 And incubating for 24h to obtain the selenium-enriched yeast with high yield of selenomethionine. The invention firstly uses the saccharomyces cerevisiae MET6 The gene modification is related to the selenium enrichment of saccharomyces cerevisiae. The content of the selenomethionine in the selenium-enriched cells of the strain is increased by at least two times compared with that of wild yeast cells. |
| priorityDate | 2017-07-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 43.