| abstract |
The invention discloses a kind of for detecting the antigen of tuberculosis infection T cell, the amino acid sequence of the antigen is as shown in SEQ ID NO.1~SEQ ID NO.28, appoint and eight polypeptides in SEQ ID NO.1~SEQ ID NO.28 is taken to cooperate with ESAT-6 and CFP-10 polypeptide and derivative collective effect, the secretion of gamma-IFN of the T cell energy specificity of differential stimulus mycobacterium tuberculosis infection, increases the sensitivity of detection.The present invention provides a kind of new detection kits that can be used for tuberculosis infection T cell, use the kit, direct human peripheral blood carries out antigenic stimulus, without separating peripheral blood mononuclear cells, detect sensitivity and specificity with higher, and it is easy to operate, testing cost is lower, clinical value with higher. |