http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107217039-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-46 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-32 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-50 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2513-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-119 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0693 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-09 |
| filingDate | 2017-08-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-03-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-03-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-107217039-B |
| titleOfInvention | Tumor tissue 3D culture method and culture solution |
| abstract | The invention discloses a tumor tissue 3D culture method suitable for various tumor tissues, which comprises the following steps: 1) dropping hydrogel into the pore plate, transferring the cut tumor tissue block into the hydrogel, dropping culture solution and CO 2 Standing in an incubator to solidify the hydrogel; 2) adding a culture solution into the pore plate for culturing, and then taking out and fixing the cultured tumor tissue block; the culture solution comprises the components of Advanced DMEM/F12 culture solution, 1 XGlutaMAX, epidermal growth factor EGF, fibroblast growth factor FGF10, N-acetyl-L-cysteine NAC, prostaglandin E2PGE2, Nicotinamide and 50 XB 27. In addition, the invention also discloses a tumor tissue 3D culture solution. The method is suitable for various tumor tissues, can evaluate the drug effect of the antitumor drug more quickly and accurately, makes up the defects of 2D cell culture and animal experiment evaluation methods, and provides a more convenient and economic model method for clinical drug research and new drug research and development. |
| priorityDate | 2017-08-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 48.