http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107202849-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-74 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-74 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-06 |
| filingDate | 2017-07-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-03-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-03-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-107202849-B |
| titleOfInvention | Method for separating and determining vitamin E and impurities in preparation of vitamin E by HPLC (high performance liquid chromatography) |
| abstract | The invention discloses a method for separating and determining vitamin E and impurities in a preparation thereof by high performance liquid chromatography, which adopts the following modes: chromatographic conditions are as follows: performing gradient elution by using octadecylsilane chemically bonded silica as a filler, using a mixed solution of acetonitrile, ethanol and water as a mobile phase A and using a mixed solution of acetonitrile, ethanol and methanol as a mobile phase B; detection wavelength: 260-310 nm; column temperature: 25-45 ℃; flow rate of mobile phase: 0.5-2.0 mL/min; sample introduction amount: 20-150 mu L; preparing a sample solution: preparing a dl-alpha-tocopherol acetate peak identification reference solution by using a polar solvent, wherein the concentration of the dl-alpha-tocopherol acetate peak identification reference solution can be 0.5-3 mg/mL; and (3) determination: and injecting the solution into a high performance liquid chromatograph, recording a chromatogram and analyzing. The method of the invention can accurately carry out quantitative analysis on the vitamin E of the raw material medicine and the related substances of the preparation thereof, thereby ensuring the quality controllability of the vitamin E and the preparation thereof. |
| priorityDate | 2017-07-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 32.