http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107190050-B
Outgoing Links
Predicate | Object |
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classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2326-12 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-28 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-28 |
filingDate | 2016-03-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2020-10-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2020-10-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-107190050-B |
titleOfInvention | HRP activity determination and H2O2Concentration detection kit and application thereof |
abstract | The invention relates to a biochemical detection kit, which comprises a reagent A: SDS/[ C 2 mim][BF 4 ]Compounding reaction liquid; and (3) reagent B: solid powder of chromogenic substrate 3,3', 5' -Tetramethylbenzidine (TMB); and (3) reagent C: h 2 O 2 And (3) solution. And may further include D: HRP enzyme solution; and (3) reagent E: buffer solution PBS, and reagent F: substrate solvent dimethyl sulfoxide (DMSO-d) 6 99.9%). The kit is based on SDS/[ C 2 mim][BF 4 ]The compound solution can obviously improve HRP-H 2 O 2 Principle of color development efficiency of TMB reaction, measurement of HRP enzyme Activity and H Using the synergistic Effect 2 O 2 And (5) detecting the concentration. The kit has the advantages of rapid color development, sensitivity and stability, the reagent for detection is biologically nontoxic, the operation is convenient and rapid, the cost is low, and the kit has the advantages of large-scale HRP activity and H 2 O 2 The commercial potential of concentration detection. |
priorityDate | 2016-03-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 25.