http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107182343-B

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filingDate 2017-05-11-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2021-01-01-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2021-01-01-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-107182343-B
titleOfInvention Method for improving endosperm activity of kalopanax septemlobus seeds after low-temperature storage
abstract A method for improving endosperm viability of kalopanax bungei seeds after cryopreservation, comprising: taking out the dried seeds stored at the low temperature of between 18 ℃ below zero and 20 ℃ below zero and the closed container for storing the seeds; standing at 15 deg.C and 15% relative humidity for 24 hr, and taking out seeds; the seeds are placed in an incubator at the temperature of 60 ℃ and are taken out after heat treatment for 6 hours. After the seeds are recovered to the room temperature, the seeds are placed on a 1% agar culture medium and are placed in a culture room at the temperature of 20 ℃ for 24 hours; cutting seed coat and endosperm of the treated seeds, soaking in 1% triphenyltetrazolium chloride (TTC) solution, and placing in dark at 30 deg.C for 48 hr; the soaked seeds are taken out and cut, the staining condition of the endosperm is observed, and the viable endosperm shows uniform red. The method can remarkably improve endosperm activity of kalopanax septemlobus seeds stored at low temperature for different time in different production areas. Solves the problem of reduced endosperm activity after low-temperature preservation of the kalopanax septemlobus seeds.
priorityDate 2017-05-11-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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