http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107177649-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-00 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-58 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02 |
| filingDate | 2017-06-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-03-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-03-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-107177649-B |
| titleOfInvention | Fermentation process for improving expression quantity of recombinant human brain natriuretic peptide fusion protein |
| abstract | The invention discloses an improvementThe fermentation process of the recombinant human brain natriuretic peptide fusion protein expression quantity comprises the following steps: adding 18-20L of BHD culture medium, 65-80 mL of glycerol and ampicillin to a final concentration of 100mg/L in a fermentation tank, inoculating, adjusting the ventilation amount to ensure that the dissolved oxygen in the fermentation liquor is 30-50%, adjusting the pH value to 6.8-7.2, and culturing at 37 ℃; when OD is reached 600 And (3) when the concentration is 10-15, adding isopropyl thiogalactoside until the final concentration is 0.5-1.0 mmol/L for induction, feeding 150-300 mL/h of feed liquid A into the fermentation tank, stopping fermentation culture after 3-6 h, and harvesting bacteria. The fermentation process of the invention obviously improves the expression level of the rhBNP fusion protein, the expression level is as high as 34.5-38.3%, and the invention has good commercial development value and has great commercial success. |
| priorityDate | 2017-06-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 51.