http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107177556-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_aaaaf4049c38c48aea4f1320fa64fb20 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-4283 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-91 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-42 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-577 |
| filingDate | 2016-03-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b5cb86328d3e359acc3b7366befa085c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_327318ae79e169feae88b39b7ddb73ab http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4838aed5debd1770d90f6e161954e7e6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_abbca8b471fb716cdec336d65ddfdd71 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_79695c9e8d7ef4320eeb62ba951bf456 |
| publicationDate | 2017-09-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-107177556-A |
| titleOfInvention | Anti- sheep IgM μ chain monoclonal antibodies, the hybridoma cell strain for secreting the antibody and application thereof |
| abstract | The invention discloses anti-sheep IgM μ chain monoclonal antibodies, the hybridoma cell strain for secreting the antibody and application thereof.The present invention is using secreting type IgM albumen as target protein, clone obtains the entire open reading frame of IgM μ chain genes, it is subcloned to prokaryotic expression carrier, utilize ni-sepharose purification recombinant protein, and female BAl BIc/c mouse of 68 week old are immunized, when antibody titer is more than 1: 10000, the single cell suspension of immune mouse spleen cell is merged with the myeloma cell of exponential phase, screening obtains the hybridoma cell strain of the monoclonal antibody of the anti-sheep IgM μ chains of secretion；To 68 week old through producing BALB/c mouse intraperitoneal injection hybridoma, inject and collect within 7 days or so ascites, protein G/A sepharose 4Bs purifying ascites obtains the monoclonal antibody of the higher anti-sheep IgM μ chains of purity.The antibody titer that the present invention is provided is high, available for the detection of sheep IgM immunoglobulins, and is expected to realize the early screening to a variety of sheep diseases and monitoring, is with a wide range of applications and social demand. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110483638-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111748038-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108384761-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110372790-A |
| priorityDate | 2016-03-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 82.