http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107164537-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0f4ecccddd7d799178f5f5662a748b90 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-124 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-158 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6888 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2017-07-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7d49784c2cac5434a1ea627d8e1042ee http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f0c7c6fa8ae44e015d75ed78f8f381a1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_86a59022949753a06efa136636ee8b2e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_140926d9a1282724735caed7fdbbf0e0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_be6e108aae0130b3557ca6ecfced2f93 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fb7949c6b72cda45d60dc646ae383d6a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_10e4f028586bf7fa9131e5771e0a77e1 |
| publicationDate | 2017-09-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-107164537-A |
| titleOfInvention | A kind of method of the gene expressions of fluorescent quantitative PCR technique detection apis mellifera major royal jelly proteins MRJP 2 |
| abstract | The invention discloses a kind of method that fluorescent quantitative PCR technique detects apis mellifera major royal jelly proteins MRJP2 gene expressions, specifically related to biological technical field, the foundation of the real-time fluorescence RT PCR detection methods of apis mellifera MRJP 2, comprises the following steps in the present invention：(1) detection and acquisition of gene are carried out using primer；(2) extraction of total serum IgE；(3) cDNA is synthesized；(4) real-time fluorescence PCR；(5) benomyl before and after the processing apis mellifera MRJP 2 relative expression quantity calculate；The real-time fluorescence RT PCR that the present invention is used are compared with Standard PCR, real-time fluorescence PCR technology collects fluorescence signal by self-reacting device, it is to avoid the subjectivity that naked eyes judge, further improve sensitivity；And RT PCR are post-processed without PCR, it is to avoid pollution, it is ensured that the reproducibility and reliability of result, real-time fluorescence RT round pcrs also exempt the follow-up tedious steps such as electrophoresis in Standard PCR, quantitative scanning, substantially reduce experimental period. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111735966-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111735966-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108384839-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111303282-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111303282-B |
| priorityDate | 2017-07-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 356.