http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107163105-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2299-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02P20-55 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K7-08 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K7-08 |
| filingDate | 2017-06-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-12-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-12-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-107163105-B |
| titleOfInvention | Crystallization method of natural linaclotide |
| abstract | The invention relates to a crystallization method of natural linaclotide, belonging to the technical field of medicine preparation. The method comprises the following steps: resin is taken as a solid phase carrier, and D-type amino acid with N-fluorenylmethyloxycarbonyl (Fmoc) protection is condensed in sequence according to a solid phase polypeptide synthesis method; after the condensation of amino acid is finished, removing side chain protecting groups and separating polypeptide from resin by using a cutting fluid, precipitating the cutting fluid by using ether to obtain crude peptide of the mirror image linaclotide, and separating and freeze-drying by using high performance liquid chromatography to obtain purified reduced linaclotide; finally, correct pairing of disulfide bonds is realized through renaturation folding, and the mirror image linaclotide with correct spatial configuration can be obtained; adding the benralite with equal concentration into a neutral buffer solution of the natural linaclotide, fully and uniformly mixing, and realizing the crystallization of the benralite with the auxiliary natural linaclotide by using different crystallization solutions, specific temperature and time by using a protein crystallization screening method. |
| priorityDate | 2017-06-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 86.