| abstract |
The invention relates to a novel protein self-assembly nano vaccine and a preparation method thereof, the novel protein self-assembly nano vaccine is prepared based on antigen protein self-assembly, a molecular adjuvant is selectively introduced in the vaccine preparation process, the antigen content is more than or equal to 85 percent, the high-efficiency immune effect can be initiated without the assistance of an aluminum adjuvant, a Freund's adjuvant and the like, the sulfydryl among protein molecules is exposed by physical regulation and control, and stable protein nanoparticles mainly crosslinked by disulfide bonds are formed through sulfydryl/disulfide bond exchange reaction. The invention overcomes the defects that the traditional nano vaccine needs to introduce an exogenous vector or a cross-linking agent and the like, and can simultaneously improve the immune effect and the biological safety of the vaccine. The obtained vaccine particles have the advantages of regular shape, strong stability, flexible regulation and control mode, good repeatability and capability of efficiently stimulating dendritic cells to mature. Has stronger universality and universality, is verified on a series of antigen proteins, and has potential important application value in the fields of novel vaccine methods and biological pharmacy. |