http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107151677-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2810-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-107 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2740-15043 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-86 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-867 |
| filingDate | 2017-03-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-02-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-02-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-107151677-B |
| titleOfInvention | Method for knocking out low transfection efficiency cell line based on CRISPR/Cas9 multiple genes |
| abstract | The invention discloses a method for efficiently knocking out cells with low transfection efficiency based on lentivirus expression CRISPR/Cas 9. The method involves two lentiviruses, one is a lentivirus expressing sgRNA and Cas9, and the other is a lentivirus expressing Cre protein. The sgRNA and Cas9 of multiple genes are simultaneously cloned between two homodromous loxps of a lentiviral vector, multiple genes are simultaneously knocked out in cells with low transfection efficiency, and then the integrated Cas9 and sgRNA in a genome are effectively removed by using a lentivirus expressing Cre, so that the off-target effect caused by over-expression of Cas9 and sgRNA is prevented. The invention combines the characteristics of high-efficiency infection of dividing and non-dividing cells and high-efficiency removal reaction of the loxp-Cre system possessed by lentivirus, and the system has the characteristics of low toxicity, high gene knockout efficiency, high accuracy, short period and the like, and has great significance for realizing multi-gene knockout in a cell line with low transfection efficiency. |
| priorityDate | 2017-03-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 27.