| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6e9b9c766d2088dd5b1d268222460654 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2510-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-107
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-22 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K38-37
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-755
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0682
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-85 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-85
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P7-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K38-37
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-755 |
| filingDate |
2017-07-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_717cc9e89dc2bc26031af8a1348577b1
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bb1dba2008f0b40a2db865b245d602c6
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_47b4a2891cca9a877292d3f4b32fd1eb
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_89d0fbe19d018824def6a8532e281469
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1107c02f24751f0bb5c1dff90343ceaa
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_460bcc52582696000a7d5a7477eb017a |
| publicationDate |
2017-09-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
CN-107142278-A |
| titleOfInvention |
A kind of method for the ripe human blood coagulation factors VIII for preparing B areas missing |
| abstract |
The invention belongs to field of biological pharmacy, it is related to screening and identification, the preparation of the ripe human blood coagulation factors VIII of B areas missing, activity calibrating and the application pharmaceutically of transfection, the H of F VIII and the L highly-expressing cell strains of F VIII of the structure, mammalian cell of the H of human blood coagulation factors VIII heavy chain F VIII and the L expression vectors of light chain F VIII.The present invention relates to the preparation method that a kind of novel B area lacks the factors of F VIII.The H genes of heavy chain F VIII for being suitable to be expressed in Chinese hamster ovary celI system and the L genes of light chain F VIII are obtained by artificial synthesis.Both are started by CMV promoter respectively, SV40Enhancer and Intron containing Enhanced expressing in expression unit, introduce GS genes and EGFP gene is easy to carry out screening and cloning to overexpression cell line.After the expression product of two genes is mixed, its mixture possesses the blood coagulation activity of the natural factors of F VIII. |
| priorityDate |
2017-07-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |