http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107142249-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K2039-552 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2710-16734 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2710-16751 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N7-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K39-12 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-245 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N7-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P31-22 |
| filingDate | 2017-05-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-01-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2018-01-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-107142249-B |
| titleOfInvention | A kind of method of full suspension cell culture production PRV antigen |
| abstract | The present invention relates to a kind of method of full suspension cell culture production PRV antigen, it comprises the following steps：1) BHK21 cells are recovered, expanded with flask suspension culture, be seeded in reactor and suspension culture is carried out using low blood serum medium, obtain suspended culture cell liquid；2) change the low blood serum medium in suspended culture cell liquid into serum free medium, continue to cultivate, connect poison and carry out Virus culture；3) continue to cultivate BHK21 96~144h of suspension cell, until dead cell quantity reaches more than 90%, harvesting suspension, freeze thawing, centrifugation, collect supernatant, produce virus liquid；4) virus liquid concentrated, inactivated, be degerming, being produced.The PRV antigen vaccine potency of gained of the invention is high, stable 10 8.5 TCID 50 / more than mL, and purity is high, steady quality, significantly improves product quality. |
| priorityDate | 2017-05-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 34.