| abstract |
The present invention relates to tumor markers detection field in medical science, colorimetric immunoassay sensor specifically is built using label, the detection to carcinomebryonic antigen is realized.A kind of colorimetric methods of quick detection carcinomebryonic antigen, prepare Ag 3 PO 4 / Ag nano composite materials are simultaneously modified carcinomebryonic antigen antibody, prepare Fe 3 O 4 / Ag nano composite materials are simultaneously modified carcinomebryonic antigen antibody, by Fe 3 O 4 @Ag‑Ab 1 Nanosphere dispersion liquid is incubated the CEA 30 minutes of various concentrations successively, is then cleaned with water 25 times, then use Ab 2 ‑Ag 3 PO 4 / Ag nanospheres dispersion liquid is incubated 30 minutes, is built into immunosensor, adds ABS buffer solutions and tetramethyl benzidine TMB solution, by UV spectrophotometer measuring TMB UV absorption come quantitative CEA concentration. |