| abstract |
The present invention relates to enzyme engineering field, specifically, a kind of phenylalanine mutase, encoding gene, recombinant vector, host cell, multiple PCR primer and their application are disclosed.The amino acid sequence of the phenylalanine mutase such as SEQ ID NO：Shown in 1.The present invention to existing phenylalanine mutase by carrying out rite-directed mutagenesis so that the phenylalanine mutase after mutation has more preferable catalytic activity, while the generative capacity of α phenylalanines can be reduced.And by setting up expression vector, recombinant bacterium is built, high efficient expression is realized, the phenylalanine mutase for meeting industrialization production requirements is obtained. |