http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107109383-B
Outgoing Links
Predicate | Object |
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classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-922 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2521-325 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y301-11001 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6848 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N1-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-34 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-55 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-22 |
filingDate | 2015-08-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2019-04-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2019-04-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-107109383-B |
titleOfInvention | thermolabile exonuclease |
abstract | The present invention provides an exonuclease or an enzymatically active fragment thereof, the exonuclease having the amino acid sequence of SEQ ID No. 1 or an amino acid sequence at least about 50% identical thereto, wherein the exonuclease or its The enzymatically active fragment (i) is substantially irreversibly inactivated by heating in a buffer consisting of 10 mM Tris-HCl, pH 8.5, 50 mM KCl and 5 mM MgCl at 25 °C for 10 minutes at a temperature of about 55 °C (ii) is substantially specific for single-stranded DNA; and (iii) has 3'-5' exonuclease activity. The present invention also provides a method for removing single-stranded DNA from a sample, a nucleic acid amplification method, a reverse transcription method, and a nucleic acid sequence analysis method in which an exonuclease or an enzymatically active fragment thereof is used. The present invention further provides a nucleic acid encoding the exonuclease or an enzymatically active fragment thereof, and a kit or composition comprising the same. |
priorityDate | 2014-08-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 135.