http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107099612-B
Outgoing Links
Predicate | Object |
---|---|
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6834 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-682 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6834 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6886 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-682 |
filingDate | 2017-06-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2020-10-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2020-10-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-107099612-B |
titleOfInvention | Method for detecting cancer patient serum miRNA based on short nucleotide chain connection |
abstract | The invention discloses a method for detecting serum miRNA of a cancer patient based on short nucleotide chain connection, which detects the mismatch and partial complementation of a nucleic acid probe and a target miRNA in hybridization through the capability of specifically recognizing and cutting unpaired single-stranded nucleic acid and base mismatch double-stranded nucleic acid; and then, the enzymatic reaction between horseradish peroxidase combined on the nucleic acid probe and a chemiluminescent substrate is utilized to generate fluorescence, a detection signal is quickly amplified, the content of the target miRNA is determined according to the fluorescence intensity, the detection result is accurate and reliable, the operation is simple and easy to implement, and the method has the advantages of low cost, high flux, no need of nucleic acid extraction and amplification and the like. |
priorityDate | 2017-06-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 17.