http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107098934-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07F9-65517 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07F9-655 |
| filingDate | 2017-04-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-03-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2019-03-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-107098934-B |
| titleOfInvention | A kind of method of phthalic aldehyde derivative phosphoramidite monomer, synthetic method and the quick coupling protein matter of DNA |
| abstract | A kind of phthalic aldehyde derivative phosphoramidite monomer, synthetic method and and the quick coupling protein matter of DNA method, be related to the quick coupling protein matter of DNA.The molecular formula of the phthalic aldehyde derivative phosphoramidite monomer is C 28 H 46 N 3 O 6 P.Phthalic aldehyde carboxylic acid derivates (OPA-COOH) are reacted with 6- amino -1- hexanol first prepares phthalic aldehyde hydroxy derivatives (OPA-OH); then in nitrogen protection and dichloromethane solvent and N; under the conditions of N- diisopropylethylamine; with 2- cyanoethyl-N; N- diisopropyl chloro phosphoramidite reacts 2h under room temperature; it is separated through silica gel column chromatography, obtains phthalic aldehyde derivative phosphoramidite monomer.The reaction characteristics for quickly, efficiently producing benzyl first lactams, the final purpose realizing OPA-DNA and native protein and being quickly coupled can be carried out with the amino on protein lysine residues using the adjacent aldehyde radical of phthalic aldehyde derivative.Compared with other coupling methods, this method has the advantages that selectively, fast and efficiently. |
| priorityDate | 2017-04-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 67.