| abstract |
The present invention provides compositions and methods for reprogramming somatic cells using purified RNA preparations comprising single-stranded mRNA encoding iPS cell-inducing factors. The purified RNA preparation is preferably substantially free of RNA contaminating molecules that: i) activate an immune response in somatic cells, ii) reduce the expression of single-stranded mRNA in somatic cells, and/or iii) activate in somatic cells RNA sensor. In certain embodiments, the purified RNA preparation is substantially free of partial mRNA, double-stranded RNA, uncapped RNA molecules, and/or single-stranded linked mRNA. |