http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107084938-B
Outgoing Links
Predicate | Object |
---|---|
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-33 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-33 |
filingDate | 2017-05-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2020-02-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2020-02-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-107084938-B |
titleOfInvention | Alkaline phosphatase determination method based on chitosan-platinum mimic oxidase |
abstract | The invention discloses an alkaline phosphatase determination method based on chitosan-platinum mimic oxidase, which is characterized in that the alkaline phosphatase is utilized to hydrolyze ascorbic acid phosphate to generate ascorbic acid, a chitosan-platinum mimic oxidase/3, 3 ', 5, 5' -tetramethylbenzidine hydrochloride catalytic color development reaction system is influenced, and the alkaline phosphatase and the content of an inhibitor thereof are directly determined by combining the change of solution color and ultraviolet absorption spectrum characteristics. In the range of 0.5-20U/L, the absorbance value A 450 The concentration of the alkaline phosphatase was linear, and the detection limit was 0.34U/L. The method is simple and convenient to operate, has high sensitivity, and can be used as an analysis method to be applied to the determination of the contents of the alkaline phosphatase and the inhibitor thereof in the environmental and life science systems. |
priorityDate | 2017-05-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 279.