http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107083361-B
Outgoing Links
Predicate | Object |
---|---|
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-599 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-998 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-2302 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-2301 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-2321 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-2307 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-15 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-38 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0646 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0783 |
filingDate | 2017-06-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2020-12-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2020-12-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-107083361-B |
titleOfInvention | Cell culture method |
abstract | The invention belongs to the field of cell culture, and particularly relates to a cell culture method. The invention provides a cell culture method, which comprises the following steps: 1) isolating peripheral blood mononuclear cells; 2) adding the isolated peripheral blood mononuclear cells of step 1) to an activating medium containing plasma and activating NK cells; 3) adding a proliferation medium to the peripheral blood mononuclear cells cultured up to day 10 in step 2) promotes proliferation of NK cells. The cell culture method provided by the invention can effectively overcome the technical defect of small amount of amplified cells in the in-vitro amplification of NK cells. |
priorityDate | 2017-06-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 93.