abstract |
The present invention relates to poultry breeding technique field, the more particularly to selection of camellia chicken.The selection of the camellia chicken includes:Extract the total protein of brain tissue corpus straitum, the protein example separated through electrophoresis;The protein example of separation is transferred to solid phase carrier;The protein on polyclonal antibody and solid phase carrier for being prepared the present invention using immunoblotting carries out immune response, detects antigen antibody complex.Antibody prepared by the present invention can recognize that immunogene, and potency is more than 1:20000;The selection for the camellia chicken that the present invention is provided can accurately select camellia chicken, be no longer limited to profile selection, can carry out early stage seed selection, shorten the seed selection cycle. |