http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107064330-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/H01J49-004 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-49 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-74 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6848 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-72 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-02 |
| filingDate | 2011-12-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-02-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-02-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-107064330-B |
| titleOfInvention | Quantification of insulin by mass spectrometry |
| abstract | The present invention describes methods for determining the amount of insulin in a sample. The present invention describes mass spectrometry methods for detecting and quantifying insulin in biological samples using purification methods combined with tandem mass spectrometry or high resolution/high precision mass spectrometry techniques. The method includes subjecting the sample to solid phase extraction (SPE) and high performance liquid chromatography (HPLC) to obtain a fraction enriched in insulin derived from the sample; (b) in a manner suitable to generate a fraction detectable by mass spectrometry; subjecting the enriched insulin to an ionization source under conditions of one or more insulin ions; (c) determining the amount of one or more insulin ions by tandem mass spectrometry, wherein the sample was not immunopurified prior to ionization. The amount of the one or more ions determined in step (c) is used to determine the amount of insulin in the sample. |
| priorityDate | 2010-12-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 144.