http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107058490-B
Outgoing Links
Predicate | Object |
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classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6869 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-04 |
filingDate | 2017-01-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2018-03-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2018-03-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-107058490-B |
titleOfInvention | Enteron aisle and the analysis method of oral cavity flora diversity and otherness |
abstract | The invention provides a kind of enteron aisle based on high throughput sequencing technologies of new generation and the analysis method of oral cavity flora diversity and otherness.This method comprises the following steps:1) collect sample to be tested and carry out microbe genome DNA extraction, obtain extracting genome DNA liquid;2) concentration and purity that detection determines microbe genome DNA in sample are carried out to extracting genome DNA liquid;3) enter performing PCR using the special V3 V4 areas primer pair extracting genome DNA liquid of 16SrDNA to expand, progress library construction, 4) it is sequenced using IlluminaMiSeq sequencing systems;5) FLASH is utilized, QIIME, usearch61 carry out bioinformatic analysis.Compared with traditional bacteria detection method, the composition and function of flora can in more detail, deeper be parsed using high-throughput techniques of new generation. |
priorityDate | 2017-01-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 17.