http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107058461-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-44 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-61 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-44 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-61 |
| filingDate | 2017-04-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-12-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-12-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-107058461-B |
| titleOfInvention | Method for measuring lipase activity and evaluating performance by taking oil-rich peel powder as substrate |
| abstract | The invention provides a method for measuring the activity and evaluating the performance of lipase by using grease-rich peel powder as a substrate. The method takes animal skin as a raw material to prepare a skin powder substrate rich in grease; in water medium, lipase contacts with oil adsorbed by peel powder to catalyze the hydrolysis of oil in peel powder to generate fatty acid, after reaction under certain condition, salt and acid are used to stop the enzyme reaction, and the fatty acid salt in bath liquid is converted into fatty acid, so that oil and fatty acid are completely adsorbed by peel powder. Completely extracting the fatty acid generated in the hide powder by using a solvent, and characterizing the activity and performance of the lipase by measuring the yield of the fatty acid in unit time. The method can measure the activity of the lipase and evaluate the performance of the lipase in a wider pH range, the used substrate and reaction conditions are similar to the conditions of the lipase acting on the intradermal grease in the animal skin treatment process, and the measurement result has more guiding significance for the selection of the lipase and the optimization of the application conditions in the lipase application fields of leather making, fur making, gum making, collagen extraction and the like which take animal skins as raw materials. |
| priorityDate | 2016-12-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 103.