http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107058266-B
Outgoing Links
Predicate | Object |
---|---|
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y302-01017 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-2462 |
classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-545 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-01 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-45 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-125 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-36 |
filingDate | 2017-04-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2019-08-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2019-08-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-107058266-B |
titleOfInvention | A method of lysozyme is prepared by zymotechnique |
abstract | The present invention relates to a kind of methods for preparing lysozyme by zymotechnique, the following steps are included: fluid nutrient medium is taken to be placed in triangular flask, thallus seed is accessed by the inoculum concentration of 1-10%v/v, carry out shake flask fermentation, fermentation condition is 20-40 DEG C, 150-300r/min, fermented and cultured for 24 hours after, fermentation liquid is centrifuged removal thallus, supernatant is taken to be purified with ammonium sulfate salting-out process, up to lysozyme, the fluid nutrient medium includes glucose, peptone and inorganic salts, and the inorganic salts are concentration than the MgSO for 1:4-4:1 4 And ZnCl 2 Mixture.The fermentation process obtains the lysozyme of greater activity by the type and concentration of inorganic salts in Optimal Medium. |
priorityDate | 2017-04-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 67.