http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107058263-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-102 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y302-01002 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-81 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-2425 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-56 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-81 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-26 |
| filingDate | 2017-01-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-11-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-11-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-107058263-B |
| titleOfInvention | Efficient preparation method of novel beta-amylase |
| abstract | The invention provides a high-efficiency preparation method of novel beta-amylase. According to the barley beta-amylase coding gene bba Base sequence and codon preference of Saccharomyces cerevisiae bba Codon optimization of gene and full-gene synthesis bbaP The gene is obtained by using non-high-fidelity DNA polymerase at low annealing temperature (45-50℃) bbaP Novel gene obtained by performing error-prone PCR bbaPT Cloning into pPIC9K vector to construct recombinant expression plasmid pPIC-bbaPT, realizing integration expression in Pichia pastoris GS115, and screening to obtain recombinant strain of beta-amylase with obviously improved optimal acting temperature. The optimal action temperature of the obtained recombinant beta-amylase is 65 ℃, the optimal action pH is 5.0, and the highest enzyme activity reaches 280U/mL. |
| priorityDate | 2017-01-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 437.