| abstract |
The invention relates to the field of cell engineering, in particular to a culture medium and a culture method for inducing pluripotent stem cells to directionally differentiate kidney cells, wherein the culture medium for inducing pluripotent stem cells to directionally differentiate kidney cells comprises 10-15g/L of DEME (Demethyl Ether) basal medium, 0.1-0.5mM of mannitol, 4-8U of heparin sodium, 1-1.5mM of L-glutamine, 0.5-0.85mM of sodium pyruvate, 0.15-0.2mM of β -mercaptoethanol, 1-3mg/L of fibronectin, 27-39ng/mL of directional differentiation induction factors and a serum substitute. |