http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107058121-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_4fcbe99de2e1bc092edc299b68dac02b |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-14 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-645 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-14 |
| filingDate | 2017-02-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b4de3fa5d18087065e4c09577508505e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_65b860dbb3481f711deee82b90f2464b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0246020a858e3b56a140f74b51ba66e1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e80c8349e5740cff409c610745fab47c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3d8f3e6c6df67c00f45b9d472fe5b547 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7dd71bad11a3a445c9785e8e088da3e7 |
| publicationDate | 2017-08-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-107058121-A |
| titleOfInvention | A kind of efficient black fungus method for preparing protoplast |
| abstract | The present invention relates to a kind of efficient black fungus method for preparing protoplast, belong to the efficient method for preparing protoplast of black fungus.Solid PDA medium is made, takes the big small bacteria blocks of 0.5cm × 0.5cm to be positioned on PDA solid mediums in superclean bench black fungus strain；Prepare liquid and add rich cultural hypha base, pour into 10mL into the solid PDA culture dishes for covering with mycelia in gnotobasis, the mycelia on scraping solid PDA medium surface prepares hypertonic solution and enzyme liquid：The mycelia that 7d is cultivated in liquid medium within is filtered, mycelia is collected, centrifugation, soft reverse 10 15 times under the microscope, are measured using blood counting chamber to be resuspended after protoplast and calculate protoplast number.Advantage is to add serine during cultural hypha, can suppress thickening for hyphal cell wall to a certain extent.Its effect is screened by Experimental comparison.By the operation of each step, specifically refinement is quantitative simultaneously, has accomplished that experiment is repeatable. |
| priorityDate | 2017-02-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 40.